Inhibition of DNA Replication of Human Papillomavirus by Artificial Zinc Finger Proteins
نویسندگان
چکیده
منابع مشابه
Inhibition of virus DNA replication by artificial zinc finger proteins.
Prevention of virus infections is a major objective in agriculture and human health. One attractive approach to the prevention is inhibition of virus replication. To demonstrate this concept in vivo, an artificial zinc finger protein (AZP) targeting the replication origin of the Beet severe curly top virus (BSCTV), a model DNA virus, was created. In vitro DNA binding assays indicated that the A...
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Previously, we reported that artificial zinc-finger proteins (AZPs) inhibited virus DNA replication in planta and in mammalian cells by blocking binding of a viral replication protein to its replication origin. However, the replication mechanisms of viruses of interest need to be disentangled for the application. To develop more widely applicable methods for antiviral therapy, we explored the f...
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Zinc finger proteins acquire DNA-binding ability by Zn (II) complexation. In the zinc finger domain of the Cys2His2 type, each finger is approximately 30 amino acid residues long and consists of a simple ββα–fold stabilized by chelation of a zinc ion with the conserved Cys2His2 residues. A zinc finger motif of Cys2His2 offers an attractive framework for the design of a novel DNA-binding protein...
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MOTIVATION Cys(2)His(2) zinc finger (ZF) proteins represent the largest class of eukaryotic transcription factors. Their modular structure and well-conserved protein-DNA interface allow the development of computational approaches for predicting their DNA-binding preferences even when no binding sites are known for a particular protein. The 'canonical model' for ZF protein-DNA interaction consis...
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Human papillomavirus (HPV) DNA replication is initiated by recruitment of the E1 helicase by the E2 protein to the viral origin. Screening of our corporate compound collection with an assay measuring the cooperative binding of E1 and E2 to the origin identified a class of small molecule inhibitors of the protein interaction between E1 and E2. Isothermal titration calorimetry and changes in prot...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 2006
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.01795-05